Identification of a novel complex between human kallikrein 2 and protease inhibitor-6 in prostate cancer tissue.

نویسندگان

  • S D Mikolajczyk
  • L S Millar
  • K M Marker
  • H G Rittenhouse
  • R L Wolfert
  • L S Marks
  • M C Charlesworth
  • D J Tindall
چکیده

Human kallikrein (hK) 2 is an arginine-selective serine protease expressed predominantly in the prostate that has an 80% sequence identity with prostate-specific antigen. Expression of hK2 is elevated in the tumor epithelium compared to benign prostate tissue. We have purified, sequenced, and identified a novel hK2 complex in prostate tissue consisting of hK2 and a serine protease inhibitor known as protease inhibitor-6 (PI-6). This 64-kDa SDS-PAGE stable complex is elevated in the tumor and is approximately 10% of total hK2. No comparable complex of prostate-specific antigen was detected. PI-6, also known as cytoplasmic antiprotease, has been characterized as an intracellular inhibitor of trypsin and chymotrypsin-like proteases, which has high homology to plasminogen activator inhibitor 1 and 2. The physiological role of PI-6 in the prostate and its relationship to hK2 and prostate cancer are under investigation.

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عنوان ژورنال:
  • Cancer research

دوره 59 16  شماره 

صفحات  -

تاریخ انتشار 1999